Recombinant:Article Title: LRBA deficiency impairs autophagy and contributes to enhanced antigen presentation and T-cell dysregulation.
Article Snippet: 1998) HeLa cells (H. sapiens) ATCC CCL-2 LCL (Lymphoblastoid cell line (H. sapiens) Generated in our laboratory using Healthy donor cells and Epstein Barr Virus supernatant N/A Reagent/resource Reference or source Identifier or catalog number MP1-specific CD4+ T cell clone Generated in the laboratory of Prof. Christian Münz, University of Zürich, Switzerland Ramos B cells ATCC CRL-1596 C57BL/6 N Lrba −/− Generated in the laboratory of Prof. Manfred Kilimann. .. Max-Planck Institute Göttingen, Germany C57BL/6N-Lrbatm1.1Kili/ Ieg RRID:IMSR_EM:14924 Naive B cells (M. musculus) From C57BL/6 N WT and Lrba−/− mice N/A Recombinant DNA pmCherry-GFP-LC3 Dr. Ian Gentle University of Freiburg, Germany N/A pClneo FLAG Promega E1841 Myc-DDK LRBA Origene RC17204 pVitro-hygro-N-mychVps34/Vps15-C-V5-his Addgene 24296 pEGFP-Atg14L Addgene 21635 pEGFP-C1-hUVRAG Addgene 24296 pClneo FLAG LRBA fragments Dr. Bernice Lo National Institute of Health, USA N/A pGFP-FYCO1 Dr. Christian Behrends University of Frankfurt, Germany N/A GFP-LC3 Prof. Christian Münz, University of Zürich, Switzerland N/A MP1-LC3 Prof. Christian Münz, University of Zürich, Switzerland N/A Antibodies Rabbit anti-LC3 Cell Signaling E5Q2K Mouse anti-Lamp1 Invitrogen MA1-205 Mouse anti-WIPI2 Abcam ab105459 Rabbit ZFYVE/DFCP1 LS-bio LS-B15646-50 Rabbit anti-GAPDH Cell Signalling 2118 Mouse anti-FLAG Sigma-Aldrich SAB4301135 Mouse anti-GFP Santa Cruz SC-9996 Mouse anti-AMPK total Cell Signaling 2793S Rabbit anti-pAMPK (T172) Cell Signaling 2535S Rabbit anti-LC3 Novus NB110-2220 Rabbit anti-LRBA Sigma-Aldrich HPA023597 Rabbit anti-Myc Cell Signaling 2278S Mouse anti-PIK3R4 Novus H00030849-M02 Rabbit anti-p62 Enzo Lifescience 5114S Mouse anti-p62 AF647 Abcam ab194721 14 EMBO reports © The Author(s) on June 27, 2025 from IP 2402:800:6390:65e4:88d:215d:4430:6344 .. Reagent/resource Reference or source Identifier or catalog number Rabbit anti-p70S6K total Cell Signaling 2708S Mouse anti-p-p70S6K (T389) Cell Signaling 9206S Mouse anti-HLA-DR Cell signaling NB-100-7785 Mouse anti-HLA-DM AF488 Santa Cruz sc-32248 AF488 Rabbit anti-FYCO1 Santa Cruz 25730-1-AP Rabbit anti-P6RP Cell signaling 4858 Rabbit anti-VPS34 Novus NB110-87320SS Mouse anti-HLA-DR FITC Invitrogen A16118 Rabbit anti-pS6RP Pacific Blue Cell signaling 8520S Anti-rabbit IgG-FITC Invitrogen A16118 Anti-rabbit AF488 Cell Signaling 4412 Anti-rabbit AF555 Cell Signaling 4413 Anti-mouse AF647 Cell Signaling 4410 HRP-linked anti-mouse Santa Cruz sc-516102 HRP-linked anti-rabbit Invitrogen 31470 HRP-linked anti-Tubulin Prointech HRP-66031 PLA probe anti-mouse minus Sigma-Aldrich DU92004 PLA probe anti-goat minus Sigma-Aldrich DU92006 PLA probe anti-rabbit plus Sigma-Aldrich DUO92101 Oligonucleotides and other sequence-based reagents gRNA for CrispR IDT N.A.
Gentle:Article Title: LRBA deficiency impairs autophagy and contributes to enhanced antigen presentation and T-cell dysregulation.
Article Snippet: 1998) HeLa cells (H. sapiens) ATCC CCL-2 LCL (Lymphoblastoid cell line (H. sapiens) Generated in our laboratory using Healthy donor cells and Epstein Barr Virus supernatant N/A Reagent/resource Reference or source Identifier or catalog number MP1-specific CD4+ T cell clone Generated in the laboratory of Prof. Christian Münz, University of Zürich, Switzerland Ramos B cells ATCC CRL-1596 C57BL/6 N Lrba −/− Generated in the laboratory of Prof. Manfred Kilimann. .. Max-Planck Institute Göttingen, Germany C57BL/6N-Lrbatm1.1Kili/ Ieg RRID:IMSR_EM:14924 Naive B cells (M. musculus) From C57BL/6 N WT and Lrba−/− mice N/A Recombinant DNA pmCherry-GFP-LC3 Dr. Ian Gentle University of Freiburg, Germany N/A pClneo FLAG Promega E1841 Myc-DDK LRBA Origene RC17204 pVitro-hygro-N-mychVps34/Vps15-C-V5-his Addgene 24296 pEGFP-Atg14L Addgene 21635 pEGFP-C1-hUVRAG Addgene 24296 pClneo FLAG LRBA fragments Dr. Bernice Lo National Institute of Health, USA N/A pGFP-FYCO1 Dr. Christian Behrends University of Frankfurt, Germany N/A GFP-LC3 Prof. Christian Münz, University of Zürich, Switzerland N/A MP1-LC3 Prof. Christian Münz, University of Zürich, Switzerland N/A Antibodies Rabbit anti-LC3 Cell Signaling E5Q2K Mouse anti-Lamp1 Invitrogen MA1-205 Mouse anti-WIPI2 Abcam ab105459 Rabbit ZFYVE/DFCP1 LS-bio LS-B15646-50 Rabbit anti-GAPDH Cell Signalling 2118 Mouse anti-FLAG Sigma-Aldrich SAB4301135 Mouse anti-GFP Santa Cruz SC-9996 Mouse anti-AMPK total Cell Signaling 2793S Rabbit anti-pAMPK (T172) Cell Signaling 2535S Rabbit anti-LC3 Novus NB110-2220 Rabbit anti-LRBA Sigma-Aldrich HPA023597 Rabbit anti-Myc Cell Signaling 2278S Mouse anti-PIK3R4 Novus H00030849-M02 Rabbit anti-p62 Enzo Lifescience 5114S Mouse anti-p62 AF647 Abcam ab194721 14 EMBO reports © The Author(s) on June 27, 2025 from IP 2402:800:6390:65e4:88d:215d:4430:6344 .. Reagent/resource Reference or source Identifier or catalog number Rabbit anti-p70S6K total Cell Signaling 2708S Mouse anti-p-p70S6K (T389) Cell Signaling 9206S Mouse anti-HLA-DR Cell signaling NB-100-7785 Mouse anti-HLA-DM AF488 Santa Cruz sc-32248 AF488 Rabbit anti-FYCO1 Santa Cruz 25730-1-AP Rabbit anti-P6RP Cell signaling 4858 Rabbit anti-VPS34 Novus NB110-87320SS Mouse anti-HLA-DR FITC Invitrogen A16118 Rabbit anti-pS6RP Pacific Blue Cell signaling 8520S Anti-rabbit IgG-FITC Invitrogen A16118 Anti-rabbit AF488 Cell Signaling 4412 Anti-rabbit AF555 Cell Signaling 4413 Anti-mouse AF647 Cell Signaling 4410 HRP-linked anti-mouse Santa Cruz sc-516102 HRP-linked anti-rabbit Invitrogen 31470 HRP-linked anti-Tubulin Prointech HRP-66031 PLA probe anti-mouse minus Sigma-Aldrich DU92004 PLA probe anti-goat minus Sigma-Aldrich DU92006 PLA probe anti-rabbit plus Sigma-Aldrich DUO92101 Oligonucleotides and other sequence-based reagents gRNA for CrispR IDT N.A.
Cell-Signaling:Article Title: LRBA deficiency impairs autophagy and contributes to enhanced antigen presentation and T-cell dysregulation.
Article Snippet: 1998) HeLa cells (H. sapiens) ATCC CCL-2 LCL (Lymphoblastoid cell line (H. sapiens) Generated in our laboratory using Healthy donor cells and Epstein Barr Virus supernatant N/A Reagent/resource Reference or source Identifier or catalog number MP1-specific CD4+ T cell clone Generated in the laboratory of Prof. Christian Münz, University of Zürich, Switzerland Ramos B cells ATCC CRL-1596 C57BL/6 N Lrba −/− Generated in the laboratory of Prof. Manfred Kilimann. .. Max-Planck Institute Göttingen, Germany C57BL/6N-Lrbatm1.1Kili/ Ieg RRID:IMSR_EM:14924 Naive B cells (M. musculus) From C57BL/6 N WT and Lrba−/− mice N/A Recombinant DNA pmCherry-GFP-LC3 Dr. Ian Gentle University of Freiburg, Germany N/A pClneo FLAG Promega E1841 Myc-DDK LRBA Origene RC17204 pVitro-hygro-N-mychVps34/Vps15-C-V5-his Addgene 24296 pEGFP-Atg14L Addgene 21635 pEGFP-C1-hUVRAG Addgene 24296 pClneo FLAG LRBA fragments Dr. Bernice Lo National Institute of Health, USA N/A pGFP-FYCO1 Dr. Christian Behrends University of Frankfurt, Germany N/A GFP-LC3 Prof. Christian Münz, University of Zürich, Switzerland N/A MP1-LC3 Prof. Christian Münz, University of Zürich, Switzerland N/A Antibodies Rabbit anti-LC3 Cell Signaling E5Q2K Mouse anti-Lamp1 Invitrogen MA1-205 Mouse anti-WIPI2 Abcam ab105459 Rabbit ZFYVE/DFCP1 LS-bio LS-B15646-50 Rabbit anti-GAPDH Cell Signalling 2118 Mouse anti-FLAG Sigma-Aldrich SAB4301135 Mouse anti-GFP Santa Cruz SC-9996 Mouse anti-AMPK total Cell Signaling 2793S Rabbit anti-pAMPK (T172) Cell Signaling 2535S Rabbit anti-LC3 Novus NB110-2220 Rabbit anti-LRBA Sigma-Aldrich HPA023597 Rabbit anti-Myc Cell Signaling 2278S Mouse anti-PIK3R4 Novus H00030849-M02 Rabbit anti-p62 Enzo Lifescience 5114S Mouse anti-p62 AF647 Abcam ab194721 14 EMBO reports © The Author(s) on June 27, 2025 from IP 2402:800:6390:65e4:88d:215d:4430:6344 .. Reagent/resource Reference or source Identifier or catalog number Rabbit anti-p70S6K total Cell Signaling 2708S Mouse anti-p-p70S6K (T389) Cell Signaling 9206S Mouse anti-HLA-DR Cell signaling NB-100-7785 Mouse anti-HLA-DM AF488 Santa Cruz sc-32248 AF488 Rabbit anti-FYCO1 Santa Cruz 25730-1-AP Rabbit anti-P6RP Cell signaling 4858 Rabbit anti-VPS34 Novus NB110-87320SS Mouse anti-HLA-DR FITC Invitrogen A16118 Rabbit anti-pS6RP Pacific Blue Cell signaling 8520S Anti-rabbit IgG-FITC Invitrogen A16118 Anti-rabbit AF488 Cell Signaling 4412 Anti-rabbit AF555 Cell Signaling 4413 Anti-mouse AF647 Cell Signaling 4410 HRP-linked anti-mouse Santa Cruz sc-516102 HRP-linked anti-rabbit Invitrogen 31470 HRP-linked anti-Tubulin Prointech HRP-66031 PLA probe anti-mouse minus Sigma-Aldrich DU92004 PLA probe anti-goat minus Sigma-Aldrich DU92006 PLA probe anti-rabbit plus Sigma-Aldrich DUO92101 Oligonucleotides and other sequence-based reagents gRNA for CrispR IDT N.A.
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